1QZL
GCATGCT + Cobalt
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-08-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8416 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 24.789, 24.789, 91.818 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.570 - 2.850 |
| R-factor | 0.24375 |
| Rwork | 0.240 |
| R-free | 0.33473 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.909 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.606 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.157 | 0.288 |
| Number of reflections | 522 | |
| <I/σ(I)> | 4.1 | 2.1 |
| Completeness [%] | 96.7 | 99.5 |
| Redundancy | 6.6 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | sodium cacodylate, Cobalt dichloride, potassium chloride, MPD, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium cacodylate | ||
| 2 | 1 | 1 | Cobalt dichloride | ||
| 3 | 1 | 1 | potassium chloride | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | sodium cacodylate | ||
| 7 | 1 | 2 | Cobalt dichloride | ||
| 8 | 1 | 2 | MPD | ||
| 9 | 1 | 2 | H2O |
