1QZ9
The Three Dimensional Structure of Kynureninase from Pseudomonas fluorescens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 68.076, 68.076, 137.976 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.700 * - 1.850 |
| R-factor | 0.15475 |
| Rwork | 0.153 |
| R-free | 0.19200 * |
| Structure solution method | MIR and MR |
| Starting model (for MR) | 1c0n |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.186 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.700 * | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.063 | 0.608 |
| Number of reflections | 29707 | |
| <I/σ(I)> | 25.4 | 2 |
| Completeness [%] | 96.8 | 92.9 |
| Redundancy | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | Jancarik, J., (1991) J. Appl. Crystallogr., 24, 409. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) |
