1QZ8

Crystal structure of SARS coronavirus NSP9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-18
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9793
Spacegroup name	P 61 2 2
Unit cell lengths	89.115, 89.115, 136.675
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.700
R-factor	0.24951
Rwork	0.248
R-free	0.27100 *
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.880
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	3.000 *	2.840
High resolution limit [Å]	2.700	2.700
Rmerge	0.056 *	0.433
Total number of observations	100668 *
Number of reflections	9345
<I/σ(I)>	9.4	1.6
Completeness [%]	99.1	99 *
Redundancy	10.8	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.2	293	Campanacci, V., (2003) Acta Crystallogr.,Sect.D, 59, 1628. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	2.0 (M)
2	1	drop	phosphate-citrate	0.1 (M)	pH4.2
3	1	drop	protein	5.8 (mg/ml)
4	1	reservoir	ammonium sulfate	1.8-2.2 (M)	pH4.0-4.5
5	1	reservoir	phosphate-citrate	0.1 (M)