1QYY

Crystal Structure of N-Terminal Domain of Human Platelet Receptor Glycoprotein Ib-alpha at 2.8 Angstrom Resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-31
Detector	ADSC QUANTUM 4
Wavelength(s)	1.071564, 1.0079675
Spacegroup name	P 1 21 1
Unit cell lengths	51.610, 113.820, 56.210
Unit cell angles	90.00, 95.21, 90.00

Refinement procedure

Resolution	15.000 - 2.800
R-factor	0.223
Rwork	0.219
R-free	0.28210 *
Structure solution method	MAD
Starting model (for MR)	Ab initio
RMSD bond length	0.007
RMSD bond angle	1.500
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.070 *	0.317 *
Total number of observations	118489 *
Number of reflections	16932	1889 *
<I/σ(I)>	8.7	2.2
Completeness [%]	94.6	72.2
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	PEG 6000, SODIUM NITRATE, SODIUM ACETATE, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	12 (%(w/w))
2	1	reservoir	sodium acetate	120 (mM)
3	1	reservoir	sodium nitrate	200 (mM)	pH5.6