1QYU
Structure of the catalytic domain of 23S rRNA pseudouridine synthase RluD
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2002-09-07 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.978795, 0.978462, 0.950037 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 75.140, 75.140, 181.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 * - 2.000 |
Rwork | 0.219 |
R-free | 0.23200 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 23.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.053 | 0.133 |
Number of reflections | 36112 | |
<I/σ(I)> | 38.3 | 9.2 |
Completeness [%] | 95.1 | 69.6 |
Redundancy | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | Del Campo, M., (2003) Acta Cryst., D59, 1871. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ethylene glycol | 25-40 (%) |