1QYN
Crystal Structure of SecB from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-03-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 84.005, 84.005, 204.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.600 * - 2.350 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fx3 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.360 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.610 | 2.500 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.045 * | 0.219 |
| Total number of observations | 465596 * | |
| Number of reflections | 33605 * | |
| <I/σ(I)> | 28.6 | 5 |
| Completeness [%] | 95.8 * | 96.7 |
| Redundancy | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | Dekker, C., (2003) J.Struct.Biol., 144, 313. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 80 (mM) | ||
| 2 | 1 | reservoir | MPD | 28 (%(v/v)) | |
| 3 | 1 | drop | protein | 10 (mg/ml) |
