1QYI
X-RAY STRUCTURE OF Q8NW41 NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ZR25.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97942, 0.97922, 0.97236 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 87.480, 87.480, 115.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.500 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.27800 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.062 | 0.146 |
| Number of reflections | 23940 | |
| Completeness [%] | 89.5 | 71.6 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 20% PEG 3350, 0.2M Lithium nitrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
