1QXO
Crystal structure of Chorismate synthase complexed with oxidized FMN and EPSP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9788, 0.9790, 0.9755, 0.8855 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.059, 124.582, 85.163 |
| Unit cell angles | 90.00, 115.15, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.000 |
| R-factor | 0.16 |
| Rwork | 0.157 |
| R-free | 0.22240 * |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.585 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SnB |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.700 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.079 | 0.383 |
| Number of reflections | 102745 | |
| <I/σ(I)> | 22.9 | 4.1 |
| Completeness [%] | 99.6 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | PEG 8000, Ethylene Glycol, HEPES, Cobalt hexamine trichloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6-10 (mg/ml) | |
| 10 | 1 | reservoir | EPSP | 1 (mM) | |
| 11 | 1 | reservoir | 12 (mM) | ||
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 0.5 (mM) | |
| 5 | 1 | drop | EDTA | 2 (mM) | |
| 6 | 1 | drop | PEG8000 | 9 (%) | |
| 7 | 1 | drop | ethlene glycol | 10 (%) | |
| 8 | 1 | drop | HEPES | 100 (mM) | pH7.5 |
| 9 | 1 | reservoir | FMN | 2 (mM) |
