1QWU

Golgi alpha-mannosidase II D341N mutant complex with 5-F-guloside

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	68.850, 109.799, 138.765
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.030
R-factor	0.151
Rwork	0.151
R-free	0.18900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hty
RMSD bond length	0.015
RMSD bond angle	1.720 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.160
High resolution limit [Å]	2.030 *	2.030
Rmerge	0.110	0.450 *
Total number of observations	476418 *
Number of reflections	68989 *
<I/σ(I)>	16.8
Completeness [%]	99.9 *	98.7 *
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7	298	van den Elsen, J.M., (2001) EMBO J., 20, 3008. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	8.5 (%)
2	1	reservoir	MPD	5 (%)
3	1	reservoir	Tris	100 (mM)	pH7.0