1QWT

Auto-inhibitory interferon regulation factor-3 (IRF3) transactivation domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Spacegroup name	P 4 21 2
Unit cell lengths	135.545, 135.545, 68.521
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 2.100
R-factor	0.195
Rwork	0.195
R-free	0.21400
Structure solution method	MIR
RMSD bond length	0.005
RMSD bond angle	1.540
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *
High resolution limit [Å]	2.100 *	2.100 *
Rmerge	0.091 *	0.512 *
Total number of observations	244162 *
Number of reflections	38291 *	1737 *
Completeness [%]	98.8 *	92.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	reservoir	ammonium phosphate	250 (mM)
3	1	reservoir	Bis-Tris	100 (mM)	pH6.0