1QWH
a covalent dimer of transthyretin that affects the amyloid pathway
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2003-03-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.630, 86.050, 63.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.880 - 1.360 |
| R-factor | 0.21571 |
| Rwork | 0.215 |
| R-free | 0.22246 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1DVQ.pdb |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.560 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 29.880 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Number of reflections | 48411 | |
| <I/σ(I)> | 24.5 | 2.8 |
| Completeness [%] | 92.4 | 96.9 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 298 | PEG 4000, 0.2 M Magnesium Nitrate , pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
