1QVV

Crystal structure of the S. cerevisiae YDR533c protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-29
Detector	MARRESEARCH
Wavelength(s)	1.004
Spacegroup name	P 21 21 21
Unit cell lengths	67.019, 93.636, 151.738
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.350
R-factor	0.228
Rwork	0.228
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	23.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.083 *	0.498 *
Total number of observations	598140 *
Number of reflections	41388
<I/σ(I)>	7.9	1.75
Completeness [%]	84.0	71.4
Redundancy	14.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.4	18 *	26% PEG 4000, 50mM Na/K phosphate buffer, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9 (mg/ml)
2	1	reservoir	PEG4000	26 (%)
3	1	reservoir	sodium potassium phosphate	50 (mM)	pH6.4