1QVE
Crystal structure of the truncated K122-4 pilin from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.184, 38.930, 37.228 |
| Unit cell angles | 66.39, 111.11, 93.74 |
Refinement procedure
| Resolution | 35.580 - 1.540 |
| R-factor | 0.14112 |
| Rwork | 0.139 |
| R-free | 0.17855 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ayz |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.468 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 1.610 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Number of reflections | 26457 | |
| <I/σ(I)> | 16.5 | 6.6 |
| Completeness [%] | 93.1 | 88.5 |
| Redundancy | 2.7 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | PEG 4000, Potassium Phosphate Monobasic, Sodium Cacodylate, Tris HCl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
