1QV8
B-DNA Dodecamer d(CGCGAATTCGCG)2 complexed with proamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 1997-07-26 |
| Detector | SIEMENS |
| Wavelength(s) | 0.71073 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.310, 40.400, 65.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.21 |
| Rwork | 0.219 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 1.900 |
| RMSD bond angle | 3.400 |
| Data reduction software | SAINT (& SADABS) |
| Data scaling software | SAINT |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 2611 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | MgCl2, cacodylate, MPD, spermine HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MgCl2 | ||
| 10 | 1 | 2 | spermine | ||
| 11 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | cacodylate | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 1 | spermine | ||
| 5 | 1 | 1 | HCl | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | MgCl2 | ||
| 8 | 1 | 2 | cacodylate | ||
| 9 | 1 | 2 | MPD |
