1QUE
X-RAY STRUCTURE OF THE FERREDOXIN:NADP+ REDUCTASE FROM THE CYANOBACTERIUM ANABAENA PCC 7119 AT 1.8 ANGSTROMS
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 300 |
| Detector technology | AREA DETECTOR |
| Collection date | 1992-10 |
| Detector | SIEMENS |
| Spacegroup name | P 65 |
| Unit cell lengths | 88.200, 88.200, 97.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.800 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| R-free | 0.21400 |
| Structure solution method | MIR |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.500 |
| Data reduction software | XDS |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 * | |
| Total number of observations | 96286 * | |
| Number of reflections | 20337 | |
| Completeness [%] | 85.0 | 34 |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20 * | SEE REFERENCE 1., pH 7.0, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10.6 (mg/ml) | |
| 10 | 1 | reservoir | sodium azide | 0.1 (%(w/v)) | |
| 11 | 1 | reservoir | MES-NaOH | 0.1 (M) | |
| 2 | 1 | drop | Tris-HCl | 4 (mM) | |
| 3 | 1 | drop | beta-OG | 1 (%(w/v)) | |
| 4 | 1 | drop | PEG6000 | 8 (%(w/v)) | |
| 5 | 1 | drop | ammonium sulfate | 8 (mM) | |
| 6 | 1 | drop | sodium azide | 0.04 (%(w/v)) | |
| 7 | 1 | drop | MES-NaOH | 0.04 (M) | |
| 8 | 1 | reservoir | PEG6000 | 20 (%(w/v)) | |
| 9 | 1 | reservoir | ammonium sulfate | 20 (mM) |
