1QU7

FOUR HELICAL-BUNDLE STRUCTURE OF THE CYTOPLASMIC DOMAIN OF A SERINE CHEMOTAXIS RECEPTOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-01-15
Detector	MARRESEARCH
Spacegroup name	P 3 2 1
Unit cell lengths	132.440, 132.440, 134.590
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 * - 2.600
R-factor	0.243
Rwork	0.243
R-free	0.27600
RMSD bond length	0.011 *
RMSD bond angle	1.510 *
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.125	0.745
Number of reflections	40467
<I/σ(I)>	4.9
Completeness [%]	95.6	74
Redundancy	3.84	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	sparse matrix method *	6.5	4 *	MPD, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	EDTA	1 (mM)
2	1	1	HEPES	25 (mM)	pH7.4
3	1	1		5-10 (mg/ml)
4	1	2	N-[2-acetamide]-2-iminodiacetic acid	0.1 (M)	pH6.5
5	1	2	MPD	15-20 (%)
6	1	2	sodium citrate	0.2 (M)