1QU3
INSIGHTS INTO EDITING FROM AN ILE-TRNA SYNTHETASE STRUCTURE WITH TRNA(ILE) AND MUPIROCIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | PRINCETON 2K |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.000, 100.000, 180.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.900 |
R-factor | 0.234 |
Rwork | 0.234 |
R-free | 0.34500 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 100.000 |
High resolution limit [Å] | 2.900 |
Rmerge | 0.099 * |
Number of reflections | 23256 |
Completeness [%] | 89.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 * | 20 * | EVAPORATION |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | IleRS | 0.050 (mM) | |
2 | 1 | drop | tRNA | 0.050 (mM) | |
3 | 1 | drop | Mupirocin | 1 (mM) | |
4 | 1 | reservoir | PEG6000 | 12 (%) | |
5 | 1 | reservoir | 0.3 (M) | ||
6 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
7 | 1 | reservoir | 100 (mM) | ||
8 | 1 | reservoir | 2 (mM) | ||
9 | 1 | reservoir | beta-octyl glucopyranoside | 0.1 (%) |