1QTY
VASCULAR ENDOTHELIAL GROWTH FACTOR IN COMPLEX WITH DOMAIN 2 OF THE FLT-1 RECEPTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-12-24 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.310, 67.010, 120.840 |
| Unit cell angles | 90.00, 118.15, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.2327 |
| Rwork | 0.233 |
| R-free | 0.28440 |
| Structure solution method | MOLECULAR REPLACEMENT IN COMBINATION WITH MULTI-CRYSTAL AVERAGING |
| Starting model (for MR) | 1vpf |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.760 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 24098 | |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 99.5 | 98 |
| Redundancy | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | Wiesmann, C., (1997) Cell, 91, 695. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 30 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 5 | 1 | reservoir | 0.02 (%) | ||
| 6 | 1 | reservoir | Tris-HCl | 0.15 (M) |
