1QTH
THE INTRODUCTION OF STRAIN AND ITS EFFECTS ON THE STRUCTURE AND STABILITY OF T4 LYSOZYME
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-10-29 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 31 |
| Unit cell lengths | 53.569, 53.569, 101.871 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| Rwork | 0.189 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 20.526 * |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.048 | 0.459 |
| Number of reflections | 23171 | |
| Completeness [%] | 85.0 * | 86.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.4 | 277 | PEG3.4K, magnesium chloride, Hepes, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | magnesium chloride | 20 (mM) | |
| 2 | 1 | 1 | HEPES | 0.1 (M) | |
| 3 | 1 | 1 | PEG3400 | 30 (%(w/v)) | |
| 4 | 1 | 1 | oxidized beta-mercaptoethanol |
