1QSU
CRYSTAL STRUCTURE OF THE TRIPLE-HELICAL COLLAGEN-LIKE PEPTIDE, (PRO-HYP-GLY)4-GLU-LYS-GLY(PRO-HYP-GLY)5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS |
Temperature [K] | 277 |
Detector technology | DIFFRACTOMETER |
Collection date | 1993-10-15 |
Detector | ENRAF-NONIUS CAD4 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 29.326, 26.573, 45.885 |
Unit cell angles | 90.00, 96.04, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.750 |
R-factor | 0.18 * |
Rwork | 0.180 |
R-free | 0.22700 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.240 * |
Data reduction software | CAD4 |
Data scaling software | MOLEN |
Phasing software | MERLOT |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 1.810 | |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 5745 * | |
Completeness [%] | 100.0 | 99.9 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 4 * | PEG 4K, tris HCl, Li2SO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | peptide | 10 (mg/ml) | |
2 | 1 | drop | PEG4000 | 15 (%) | |
3 | 1 | drop | Tris-HCl | 0.1 (M) | |
4 | 1 | drop | 0.3 (M) | ||
5 | 1 | reservoir | Tris | 0.1 (M) | |
6 | 1 | reservoir | 0.6 (M) | ||
7 | 1 | reservoir | PEG4000 | 30 (%) |