1QST

CRYSTAL STRUCTURE OF TETRAHYMENA GCN5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	CCD
Collection date	1998-04-29
Detector	ADSC QUANTUM 4
Spacegroup name	P 32 2 1
Unit cell lengths	64.100, 64.100, 97.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.700
R-factor	0.244
Rwork	0.244
R-free	0.27800
RMSD bond length	0.006
RMSD bond angle	1.292
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	PHASES
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.780
High resolution limit [Å]	1.700	1.700
Rmerge	0.045	0.149
Total number of observations	497452 *
Number of reflections	25427 *
<I/σ(I)>	9.4
Completeness [%]	97.3	86.3
Redundancy	19.6	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	4 *	LITHIUM SULFATE, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5	4 *	LITHIUM SULFATE, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4.5 (mg/ml)
2	1	drop		0.5 (M)
3	1	drop	HEPES	40 (mM)
4	1	reservoir		1.25 (M)
5	1	reservoir	HEPES	100 (mM)