1QSR
CRYSTAL STRUCTURE OF TETRAHYMENA GCN5 WITH BOUND ACETYL-COENZYME A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-07 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.380, 64.380, 97.450 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.26100 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.335 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.060 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.054 | 0.182 |
| Total number of observations | 158305 * | |
| Number of reflections | 16255 | |
| <I/σ(I)> | 21.1 | |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 9.7 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 25 * | HEPES, AMMONIUM SULFATE, MAGNESIUM SULFATE, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | drop | acetyl-CoA | 1.5 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 1.5 (M) | |
| 4 | 1 | reservoir | HEPES | 50 (mM) | |
| 5 | 1 | reservoir | 50 (mM) |
