1QSP

CRYSTAL STRUCTURE OF THE YEAST PHOSPHORELAY PROTEIN YPD1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1997-11-20
Detector	RIGAKU RAXIS II
Spacegroup name	P 43 21 2
Unit cell lengths	52.710, 52.710, 244.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.700
R-factor	0.206
Rwork	0.201
R-free	0.25400
RMSD bond length	0.007 *
RMSD bond angle	1.664
Data reduction software	XFIT
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.066	0.078
Number of reflections	9674 *
<I/σ(I)>	9.7
Completeness [%]	91.6	82
Redundancy	5.4	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	Xu, Q., (1999) Acta Crystallogr., Sect.D, 55, 291. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	30-33 (%(w/v))
2	1	reservoir	sodium acetate	0.1 (M)
3	1	reservoir	ammonium acetate	0.2 (M)
4	1	drop	protein	10-12 (mg/ml)