1QSJ

N-TERMINALLY TRUNCATED C3DG FRAGMENT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-07-16
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	99.620, 99.930, 114.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.000 - 1.900
R-factor	0.212
Rwork	0.212
R-free	0.25300
RMSD bond length	0.010
RMSD bond angle	1.940
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	2.170
High resolution limit [Å]	1.940	1.940
Rmerge	0.045	0.083
Number of reflections	64362	18274 *
<I/σ(I)>	12.3
Completeness [%]	76.0	77.1
Redundancy	2.8	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	15 *	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	Tris-HCl	0.01 (M)
3	1	reservoir	PEG6000	25-30 (%(w/v))
4	1	reservoir	potassium phosphate	0.05 (M)