1QSD
RBL2P, BETA-TUBULIN BINDING POST-CHAPERONIN COFACTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 289 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-01-01 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 45.400, 110.100, 49.400 |
| Unit cell angles | 90.00, 106.20, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.211 |
| Rwork | 0.208 |
| R-free | 0.29700 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.668 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | SHELXS |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.061 | 0.152 |
| Number of reflections | 11128 | |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 96.2 | 96.1 |
| Redundancy | 2.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | 293 | 1.6 M NA-CITRATE/CITRIC ACID, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | trisodium citrate/citric acid | 1.6 (M) |
