1QS1
CRYSTAL STRUCTURE OF VEGETATIVE INSECTICIDAL PROTEIN2 (VIP2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 1997-12-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.900, 78.700, 105.900 |
| Unit cell angles | 108.65, 89.96, 90.02 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.26900 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.046 | 0.270 |
| Total number of observations | 468627 * | |
| Number of reflections | 215237 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 83.4 | 69.2 |
| Redundancy | 2.2 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 20 * | PEG200, BOG, IMIDAZOLE, MALATE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | |
| 3 | 1 | reservoir | PEG200 | 30 (%(w/v)) | |
| 4 | 1 | reservoir | octyl-beta-D-glucopyranoside | 1 (%(w/v)) | |
| 5 | 1 | reservoir | imidazole/malate | 200 (mM) |
