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CRYSTAL STRUCTURE OF WILD-TYPE ALPHA-LYTIC PROTEASE AT 1.6 A, PH 5.14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	97
Detector technology	IMAGE PLATE
Collection date	1998-07-15
Detector	RIGAKU RAXIS IV++
Spacegroup name	P 32 2 1
Unit cell lengths	65.627, 65.627, 79.460
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 1.600
R-factor	0.165
Rwork	0.165
R-free	0.16900
RMSD bond length	0.009
RMSD bond angle	1.569
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.720	1.640
High resolution limit [Å]	1.600	1.600
Rmerge	0.123	0.375
Number of reflections	26626
<I/σ(I)>	15.9
Completeness [%]	100.0	99.9
Redundancy	6.88	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.14	298	LITHIUM SULFATE, TRIS-SULFATE, pH 5.14, VAPOR DIFFUSION, HANGING DROP, temperature 298K