1QRI
X-RAY STRUCTURE OF THE DNA-ECO RI ENDONUCLEASE COMPLEXES WITH AN E144D MUTATION AT 2.7 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS-NICOLET X100 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 118.400, 118.400, 49.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| Rwork | 0.164 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.496 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.600 | 2.780 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.072 | 0.133 |
| Number of reflections | 7508 | |
| Completeness [%] | 61.0 | 10 |
| Redundancy | 7 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | PRECIPITANT: 40% PEG 400; 40 MM BTP; PH 8.0; RESERVOIR: 16% PEG 400; 40 MM BTP; PH 6.5; DROP: 5 UL PROTEIN, 2 UL DNA, 1 UL PPT, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 2 | 1 | 1 | BTP | ||
| 3 | 1 | 1 | PPT | ||
| 4 | 1 | 2 | PEG 400 | ||
| 5 | 1 | 2 | BTP |
