1QRD
QUINONE REDUCTASE/FAD/CIBACRON BLUE/DUROQUINONE COMPLEX
Experimental procedure
| Detector | SIEMENS |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 72.000, 107.000, 88.400 |
| Unit cell angles | 90.00, 92.60, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.400 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 3.120 |
| Data reduction software | XDS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.050 * |
| Number of reflections | 23775 |
| Completeness [%] | 89.8 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.45 (M) | |
| 3 | 1 | reservoir | PEG8000 | 0.5 (%) | |
| 4 | 1 | reservoir | Cibacron blue(pure ring A o-sulfonate) | 30 (mM) | |
| 5 | 1 | reservoir | imidazole | 150 (mM) |
