1QQH
2.1 A CRYSTAL STRUCTURE OF THE HUMAN PAPILLOMAVIRUS TYPE 18 E2 ACTIVATION DOMAIN
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 1997-12-20 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 76.240, 76.240, 158.350 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.256 |
Rwork | 0.256 |
R-free | 0.29700 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.170 |
High resolution limit [Å] | 2.000 | 2.100 |
Rmerge | 0.052 | 0.437 |
Total number of observations | 135695 * | |
Number of reflections | 16635 | |
<I/σ(I)> | 28.9 | |
Completeness [%] | 99.0 | |
Redundancy | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.2 * | 277 | AMMONIUM PHOSPHATE, TRIS, pH 4.0, VAPOR DIFFUSION, temperature 277.0K |
1 | Vapor diffusion, hanging drop * | 8.2 * | 277 | AMMONIUM PHOSPHATE, TRIS, pH 4.0, VAPOR DIFFUSION, temperature 277.0K |
1 | Vapor diffusion, hanging drop * | 8.2 * | 277 | AMMONIUM PHOSPHATE, TRIS, pH 4.0, VAPOR DIFFUSION, temperature 277.0K |
1 | Vapor diffusion, hanging drop * | 8.2 * | 277 | AMMONIUM PHOSPHATE, TRIS, pH 4.0, VAPOR DIFFUSION, temperature 277.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8-12 (mg/ml) | |
2 | 1 | drop | 100 (mM) | ||
3 | 1 | drop | Tris-HCl | 25 (mM) | |
4 | 1 | drop | glycerol | 5 (%) | |
5 | 1 | drop | dithiothreitol | 10 (mM) | |
6 | 1 | reservoir | ammonium phosphate | 1.2-1.4 (M) | |
7 | 1 | reservoir | Tris-HCl | 100 (mM) |