1QPF

FK506 BINDING PROTEIN (12 KDA, HUMAN) COMPLEX WITH L-709,858

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1992-03-04
Detector	SIEMENS
Spacegroup name	P 61 2 2
Unit cell lengths	74.260, 74.260, 236.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.2436 *
Rwork	0.243
R-free	0.30960 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PROTEIN PORTION OF PDB ENTRY 1FKD
RMSD bond length	0.009
RMSD bond angle	1.500
Data scaling software	X-GEN
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.400
High resolution limit [Å]	2.260	2.260
Rmerge	0.045	0.193
Number of reflections	13529
<I/σ(I)>	26.63	2.41
Completeness [%]	71.2	33
Redundancy	3.86	1.54

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.6		AMMOMIUM SULFATE, BETA-HEPTYL- D-GLUCOPYRANOSIDE, POTASSIUM PHOSPHATE, pH 5.6
1	Vapor diffusion *	5.6		AMMOMIUM SULFATE, BETA-HEPTYL- D-GLUCOPYRANOSIDE, POTASSIUM PHOSPHATE, pH 5.6
1	Vapor diffusion *	5.6		AMMOMIUM SULFATE, BETA-HEPTYL- D-GLUCOPYRANOSIDE, POTASSIUM PHOSPHATE, pH 5.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	47 (%sat)
2	1	reservoir	beta-heptyl D-glucopyranoside	0.2 (%)
3	1	reservoir	potassium phosphate	0.10 (M)