1QP5
BASE-PAIRING SHIFT IN A DODECAMER CONTAINING A (CA)N TRACT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 258 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS |
| Spacegroup name | H 3 |
| Unit cell lengths | 65.900, 65.900, 47.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.600 |
| R-factor | 0.161 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | ULTIMA |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.062 |
| Number of reflections | 1603 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 277 | WATER, SPERMINE, CACODYLATE, MAGNESIUM ACETATE, pH 6.0, VAPOR DIFFUSION at 277 K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SPERMINE | ||
| 2 | 1 | 2 | CACODYLATE | ||
| 3 | 1 | 3 | MAGNESIUM ACETATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | cacodylate | 50 (mM) | |
| 2 | 1 | drop | dodecamer duplex | 1 (mM) | |
| 3 | 1 | drop | spermine tetrachloride | 1.2 (mM) | |
| 4 | 1 | drop | magnesium acetate | 16 (mM) | |
| 5 | 1 | drop | 2,4-methypentanediol | 45 (%) |
