1QOV

PHOTOSYNTHETIC REACTION CENTER MUTANT WITH ALA M260 REPLACED WITH TRP (CHAIN M, A260W)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	298
Detector technology	CCD
Collection date	1998-03-15
Detector	ADSC CCD
Spacegroup name	P 31 2 1
Unit cell lengths	142.050, 142.050, 186.810
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.100
Rwork	0.169
R-free	0.18600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	WILD-TYPE RHODOBACTER SPHAEROIDES COORDINATES (UNPUBLISHED DATA)
RMSD bond length	0.011
RMSD bond angle	0.026
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.057	0.266
Number of reflections	124853
Completeness [%]	97.6	78.6
Redundancy	4.2	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8		pH 8.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	reaction center	10 (mg/ml)
2	1	drop	LDAO	0.1 (%(v/v))
3	1	drop	1,2,3-heptanetriol	3.5 (%(w/v))
4	1	drop	trisodium citrate	0.5 (M)
5	1	drop	Tris-HCl	10 (mM)
6	1	reservoir	trisodium citrate	1.1 (M)