1QO5
Fructose 1,6-bisphosphate Aldolase from Human Liver Tissue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 291.100, 489.840, 103.360 |
| Unit cell angles | 90.00, 103.68, 90.00 |
Refinement procedure
| Resolution | 29.000 - 2.500 |
| Rwork | 0.224 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ald |
| RMSD bond length | 0.022 |
| RMSD bond angle | 0.063 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.154 | 0.567 |
| Number of reflections | 339612 | |
| <I/σ(I)> | 3.2 | 0.4 |
| Completeness [%] | 71.0 | 55.8 |
| Redundancy | 2.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | HANGING DROP WITH AMMONIUM SULFATE AS THE PRECIPITANT, pH 7.00 |
