1QO3
Complex between NK cell receptor Ly49A and its MHC class I ligand H-2Dd
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-04-15 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.963, 96.957, 99.318 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1B6E 1ddh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 24.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.9) |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.053 * | 0.161 * |
| Total number of observations | 128909 * | |
| Number of reflections | 33230 | |
| <I/σ(I)> | 15.14 | 7 |
| Completeness [%] | 94.7 | 80.7 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 4 * | Ly49A NKD : H-2Dd = 1.5:1 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Ly49A NKD | 9 (mg/ml) | |
| 2 | 1 | drop | H-2Dd | ||
| 3 | 1 | drop | HEPES | 20 (mM) | |
| 4 | 1 | drop | 100 (mM) | ||
| 5 | 1 | reservoir | mPEG550 | 9 (%) | |
| 6 | 1 | reservoir | magnesium acetate | 50 (mM) | |
| 7 | 1 | reservoir | MES | 100 (mM) |
