1QO0
Amide receptor of the amidase operon of Pseudomonas aeruginosa (AmiC) complexed with the negative regulator AmiR.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 308.440, 67.150, 76.410 |
| Unit cell angles | 90.00, 103.33, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| Rwork | 0.186 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pea |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.034 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.600 | 2.360 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 67099 | |
| <I/σ(I)> | 6.8 | 3 |
| Completeness [%] | 83.8 | 69.3 |
| Redundancy | 2.7 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.6 | CRYSTALLISATION WAS BY MICRO-BATCH AND STREAK SEEDING. WELLS CONTAINED 8-8.5% PEG4000, 20%(V/V) 2-PROPANOL, 50MM SODIUM CITRATE BUFFERED AT PH 5.6 AND AMIC-AMIR COMPLEX AT 5MG/ML (FINAL CONCENTRATION). |
