1QLN
STRUCTURE OF A TRANSCRIBING T7 RNA POLYMERASE INITIATION COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | BRANDEIS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 221.200, 73.600, 80.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.400 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cez |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.078 | 0.370 |
| Number of reflections | 51537 * | |
| <I/σ(I)> | 10.6 | 1.6 |
| Completeness [%] | 97.8 | 95.7 |
| Redundancy | 3.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8.7 | pH 8.70 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | T7 RNAP | 0.3 (mM) | |
| 2 | 1 | 1 | T7 promoter | 0.35 (mM) | |
| 3 | 1 | 1 | ribonucleotides GTP | 4 (mM) | |
| 4 | 1 | 1 | uridine 5'-triphosphate | 4 (mM) | |
| 5 | 1 | 1 | cytidine 5'-triphosphate | 4 (mM) | |
| 6 | 1 | 1 | alpha, beta-methylene ATP | 4 (mM) | |
| 7 | 1 | 1 | 15 (mM) |
