1QLB
respiratory complex II-like fumarate reductase from Wolinella succinogenes
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 275 |
| Detector technology | CCD |
| Collection date | 1999-03-26 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 118.400, 85.050, 188.850 |
| Unit cell angles | 90.00, 96.46, 90.00 |
Refinement procedure
| Resolution | 38.870 - 2.330 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1QLA |
| RMSD bond length | 0.007 |
| RMSD bond angle | 21.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.870 | 2.390 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.075 * | 0.388 * |
| Number of reflections | 152939 | |
| <I/σ(I)> | 9.5 | 2.8 |
| Completeness [%] | 95.8 | 99.7 |
| Redundancy | 2.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.4 * | pH 6.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9.5 (mg/ml) | |
| 10 | 1 | drop | malonate | 1 (mM) | |
| 11 | 1 | drop | HEPES | 10 (mM) | |
| 12 | 1 | drop | citrate | 10 (mM) | |
| 13 | 1 | reservoir | 150 (mM) | ||
| 14 | 1 | reservoir | PEG3350 | 10 (%) | |
| 15 | 1 | reservoir | citrate | 20 (mM) | |
| 2 | 1 | drop | dodecyl-beta-maltoside | 0.05 (%) | |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | drop | decylmaltoside | 0.20 (%) | |
| 5 | 1 | drop | benzamidine | 2.4 (%) | |
| 6 | 1 | drop | PEG3350 | 10 (%) | |
| 7 | 1 | drop | 75 (mM) | ||
| 8 | 1 | drop | DMF | 5 (%) | |
| 9 | 1 | drop | DMN | 1 (mM) |
