1QKK

Crystal structure of the receiver domain and linker region of DctD from Sinorhizobium meliloti

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	1998-11-27
Detector	MARRESEARCH
Wavelength(s)	0.98333, 0.98100, 0.98076
Spacegroup name	I 2 2 2
Unit cell lengths	58.651, 58.769, 167.888
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.420 - 1.700
R-factor	0.218
Rwork	0.218
R-free	0.23100 *
Structure solution method	MAD
RMSD bond length	0.008 *
RMSD bond angle	21.100 *
Data reduction software	HKL-2000 ((DENZO))
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.059 *
Number of reflections	32439 *
<I/σ(I)>	34	2.2
Completeness [%]	86.1 *	100
Redundancy	8	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6	294 *	50 MM NA SUCCINATE PH 5.6, 70 MM AMMONIUM PHOSPHATE MONOBASIC, 1 MM DTT

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-7 (mg/ml)
2	1	reservoir	succinate	50 (mM)
3	1	reservoir		75 (mM)