1QK5
TOXOPLASMA GONDII HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE WITH XMP, PYROPHOSPHATE AND TWO MG2+ IONS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-02-15 |
| Detector | PRINCETON SCIENTIFIC INSTRUMENTS 2K CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 55.208, 112.255, 144.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.000 - 1.600 |
| Rwork | 0.234 |
| R-free | 0.26099 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qk3 SUBUNIT A WITHOUT LOOPS WATERS OR GMP |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.040 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.076 * | 0.373 * |
| Total number of observations | 245248 * | |
| Number of reflections | 58200 | |
| <I/σ(I)> | 10.3 | 2.4 |
| Completeness [%] | 98.0 | 93.9 |
| Redundancy | 4.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | 4 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-20 (mg/ml) | |
| 2 | 1 | drop | IMP | 1 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 15-20 (%) | |
| 4 | 1 | reservoir | potassium phosphate | 100 (mM) | |
| 5 | 1 | reservoir | octyl-beta-D-glucopyranoside | 0.25 (%) |
