1QJQ
FERRIC HYDROXAMATE RECEPTOR FROM ESCHERICHIA COLI (FHUA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-08-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 |
| Unit cell lengths | 172.100, 172.100, 87.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.950 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qff |
| RMSD bond length | 0.008 |
| RMSD bond angle | 26.200 * |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS (0.4) |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.990 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.064 | 0.291 * |
| Total number of observations | 231385 * | |
| Number of reflections | 31400 * | |
| <I/σ(I)> | 20.26 | 4.03 |
| Completeness [%] | 94.8 | 82 * |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 18 * | equal volume of protein and reservoir solution were used for the drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | N,N-dimethyldecylamine-N-oxide | 0.80 (%) | |
| 3 | 1 | drop | ammonium acetate | 10 (mM) | |
| 4 | 1 | reservoir | mPEG2000 | 12 (%) | |
| 5 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
| 6 | 1 | reservoir | glycerol | 20 (%) | |
| 7 | 1 | reservoir | cis-inositol | 1 (%) |
