1QJP

HIGH RESOLUTION STRUCTURE OF THE OUTER MEMBRANE PROTEIN A (OMPA) TRANSMEMBRANE DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-04-15
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	65.100, 79.700, 50.200
Unit cell angles	90.00, 94.30, 90.00

Refinement procedure

Resolution	12.000 - 1.650
Rwork	0.155
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bxw
RMSD bond length	0.008
RMSD bond angle	2.500 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.750
High resolution limit [Å]	1.650	1.650
Rmerge	0.061 *	0.310 *
Number of reflections	29702
<I/σ(I)>	13.7	3.2
Completeness [%]	95.4	94 *
Redundancy	2.5	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6 *		10% PEG 8000, 10 % MPD, 25 MM KH2PO4 PH 5.1

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	C8E4	0.6 (%(w/v))
2	1	reservoir	ammonium sulfate	1.4 (M)
3	1	reservoir	2-propanol	3.8 (%(v/v))
4	1	reservoir	C8E4	0.5 (%(w/v))
5	1	reservoir	hexyldimethylaminoxide	1.8 (%(w/v))
6	1	reservoir	ammonium acetate	0.1 (M)