1QJC
Phosphopantetheine Adenylyltransferase from Escherichia coli in complex with 4'-phosphopantetheine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 135.331, 135.331, 135.331 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.670 - 1.630 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b6t |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.044 | 0.409 |
| Number of reflections | 33838 | |
| <I/σ(I)> | 44.2 | |
| Completeness [%] | 86.3 | 51.5 |
| Redundancy | 20.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | pH 5.00 |
