1QIU

A triple beta-spiral in the adenovirus fibre shaft reveals a new structural motif for biological fibres

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	1998-09-05
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	165.510, 95.870, 211.770
Unit cell angles	90.00, 106.83, 90.00

Refinement procedure

Resolution	25.000 - 2.400
R-factor	0.232
Rwork	0.232
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qhv
RMSD bond length	0.006
RMSD bond angle	26.200 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS (0.4)
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.132 *	0.287 *
Number of reflections	103327	10696 *
<I/σ(I)>	4.1	2.4
Completeness [%]	84.0	59.9 *
Redundancy	2.7	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4	20 *	0.8 M AMMONIUM SULPHATE, 100 MM SODIUM CITRATE, PH 4.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14 (mg/ml)
2	1	reservoir	ammonium sulfate	0.8 (M)
3	1	reservoir	sodium citrate	0.1 (M)