1QIO

SPECIFIC CHEMICAL AND STRUCTURAL DAMAGE CAUSED BY INTENSE SYNCHROTRON RADIATION TO HEN EGG WHITE LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	1999-05-15
Detector	ADSC QUANTUM 4
Spacegroup name	P 43 21 2
Unit cell lengths	76.929, 76.929, 37.207
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.200
R-factor	0.141 *
R-free	0.18700 *
Structure solution method	OTHER
Starting model (for MR)	194l
RMSD bond length	0.013 *
RMSD bond angle	0.031 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.240
High resolution limit [Å]	1.200	1.200
Rmerge	0.061 *	0.176 *
Number of reflections	34095
<I/σ(I)>	23.7	4.3
Completeness [%]	95.3	69.2
Redundancy	4.11	2.89

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.7		CRYSTALLIZED FROM 2-6% NACL, 0.2 M SODIUM ACETATE PH 4.7.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1		2-6 (%)
2	1	1	Na acetate	0.2 (M)