1QIB
CRYSTAL STRUCTURE OF GELATINASE A CATALYTIC DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS |
| Synchrotron site | SRS |
| Temperature [K] | 293 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 84.830, 84.830, 57.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.205 * |
| Rwork | 0.204 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.760 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.130 * |
| Number of reflections | 5551 |
| Completeness [%] | 99.5 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | pH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | reservoir | 0.010 (mM) | ||
| 4 | 1 | reservoir | 10 (mM) | ||
| 5 | 1 | reservoir | PEG8000 |
