1QI7

THE CRYSTAL STRUCTURE AT 2.0 A OF SAPORIN SO6, A RIBOSOME INACTIVATING PROTEIN FROM SAPONARIA OFFICINALIS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	309
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 43 2 2
Unit cell lengths	67.530, 67.530, 119.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.000
Rwork	0.182
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1paf
RMSD bond length	0.012
RMSD bond angle	2.240
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.230
High resolution limit [Å]	2.000	2.000
Rmerge	0.109 *	0.294
Total number of observations	210203 *
Number of reflections	19206
Completeness [%]	98.5	96.7
Redundancy	8.7	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.7	309 *	pH 4.7

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	reservoir solution
3	1	drop	PEG400	10 (%(w/v))
4	1	reservoir	ammonium sulfate	2.8 (M)
5	1	reservoir	sodium citrate	0.1 (M)