1QHY

CHLORAMPHENICOL PHOSPHOTRANSFERASE FROM STREPTOMYCES VENEZUELAE IN COMPLEX WITH ATPGAMMAS AND CHLORAMPHENICOL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Detector technology	IMAGE PLATE
Spacegroup name	I 41 3 2
Unit cell lengths	200.000, 200.000, 200.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.233
Rwork	0.233
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qhn
RMSD bond length	0.010
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.600
Rmerge	0.061	0.367 *
Total number of observations	304201 *
Number of reflections	21331
<I/σ(I)>	16.2
Completeness [%]	99.7 *	99.8 *
Redundancy	14.26 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5		pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.5 (mM)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop	ammonium sulfate	0.4 (M)
4	1	drop	magnesium chloride	10 (mM)
5	1	drop	ATPgammaS	10 (mM)
6	1	reservoir	Tris-HCl	0.1 (M)
7	1	reservoir	ammonium sulfate	1.14 (M)