1QHT

DNA POLYMERASE FROM THERMOCOCCUS SP. 9ON-7 ARCHAEON

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	98
Detector technology	IMAGE PLATE
Collection date	1997-10-15
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	95.300, 98.700, 112.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.250 *
R-factor	0.245
Rwork	0.245
R-free	0.30800 *
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	1.260 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.100
Rmerge	0.055 *
Total number of observations	652420 *
Number of reflections	58956
Completeness [%]	94.2
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	9	291	MICRODIALYSIS AT 291 K: 4-7% PEG 1500, 10 MM NICL2, 100 MM GLYCINE-NAOH, PH 9.0 OR 9.1; 25 MICROLITERS OF 3.9 MG/ML PROTEIN

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	3.9 (mg/ml)
2	1	2	glycine-NaOH	100 (mM)
3	1	2		10 (mM)
4	1	2	PEG1500	4-7 (%)