1QHJ
X-RAY STRUCTURE OF BACTERIORHODOPSIN GROWN IN LIPIDIC CUBIC PHASES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-12-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 |
| Unit cell lengths | 60.800, 60.800, 110.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.000 - 1.900 |
| R-factor | 0.224 * |
| Rwork | 0.224 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ap9 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 17.720 * |
| Data reduction software | XDS |
| Data scaling software | CCP4 |
| Phasing software | BASED (ON PACKING CONSIDERATIONS NO SPECIAL SOFTWARE) |
| Refinement software | CNS (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.046 * | 0.287 * |
| Total number of observations | 97807 * | |
| Number of reflections | 17996 | |
| <I/σ(I)> | 9.9 | 2.6 |
| Completeness [%] | 99.5 | 98.3 |
| Redundancy | 5.4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.6 | 20 * | Landau, E.M., (1996) Proc.Natl.Acad.Sci.USA., 93, 14532. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | sodium potassium Pi | 3.3 (M) | |
| 2 | 1 | 1 | protein | 3.5 (mg/ml) | |
| 3 | 1 | 1 | methylpentandiol | 0.05 (%) | |
| 4 | 1 | 1 | beta-octylglycopyranoside | 1.2 (%) |
